protein patterns and larvicide activity of crystalline inclusions of bacillus thuringiensis ssp. kumamotoensis dsm 6070

نویسندگان

j. kutasi

biofil ltd., budapest, hungary. r. kovacs

biofil ltd., budapest, hungary. i. puspan

biofil ltd., budapest, hungary. j. makk

department of microbiology, eötvös loránd university, budapest, hungary. k. takacs

چکیده

morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures of b. thuringiensis ssp. kumamotoensis dsm 6070 (bt 6070) was conducted via phase contrast and scanning electron microscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. the activity of the spore-crystal suspensions against house fly (musca domestica) larvae was also assessed. bipyramidal and smaller, irregular shaped crystals were observed in the sporulated cultures. 130, 75 and 25 kda bands were detected in the protein pattern. the presence of 25 kda proteins in bt6070 has not been reported earlier. the spore-crystal suspension showed significant larvicide activity against housefly larvae. larvicide activity of b. thuringiensis ssp. kumamotoensis against any dipteran species has not been detected yet. further studies are needed on identifying the dipteran- active fraction.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Crystalline inclusions in Bacillus thuringiensis.

Crystalline inclusion bodies resembling those seen in Clostridium cochlearium were detected in cultures of Bacillus thuringiensis infected with bacteriophage.

متن کامل

Differentiated pattern of protein composition of crystalline inclusions of newly isolated Bacillus thuringiensis strains from Silesia in Poland.

Protein profiles of crystal delta-endotoxins were determined in twenty nine Bacillus thuringiensis strains-soil and phylloplane isolates--from Poland. Electrophoretic analysis revealed quantatively and qualitatively different patterns of delta-endotoxin crystal preparations of these B. thuringiensis strains. The crystalline parasporal inclusions of B. thuringiensis isolates were composed of two...

متن کامل

Protein turnover and the formation of protein inclusions during sporulation of Bacillus thuringiensis.

During sporulation of Bacillus thuringiensiS, a spore and a protein parasporal inclusion are formed within each cell (Hannay & Fitz-James, 1955; Hannay, 1956). The parasporal inclusions are of a regular, dipyramidal form and are referred to as 'crystals'. Similar 'crystals' are formed by a number of related strains of Bacillus, all of which are pathogenic to insect larvae. The crystals are comp...

متن کامل

antioxidant and antimicrobial activity of allium jesdianum extract

چکیده بررسی فعالیت ضد اکسیدانی و ضدمیکروبی عصاره گیاه بن سرخ (allium jesdianum) به کوشش: زهرا مقیمی هدف از این تحقیق بررسی میزان ترکیبات فنولی کل، ترکیبات فلاوونوئیدی کل، فعالیت ضداکسیدانی و ضدمیکروبی عصاره ی گیاه تازه و خشک بن سرخ بود. استخراج عصاره به روش های غوطه وری، استفاده از همزن مغناطیسی، دستگاه مایکروویو، امواج فراصوت و گرمادهی مقاومتی و با استفاده از متانول، اتانول و آب به عنوا...

Regulation by overlapping promoters of the rate of synthesis and deposition into crystalline inclusions of Bacillus thuringiensis delta-endotoxins.

During sporulation, Bacillus thuringiensis produces intracellular, crystalline inclusions comprised of a mixture of protoxins active on insect larvae. A major class of these protoxin genes, designated cry1, is transcribed from two overlapping promoters (BtI and BtII) utilizing RNA polymerase containing sporulation sigma factors sigma(E) and sigma(K), respectively. Fusions of these promoters to ...

متن کامل

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of agricultural science and technology

جلد ۱۸، شماره Supplementary Issue، صفحات ۱۹۴۵-۱۹۵۱

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023